One clade (bottom of tree, Figure 4) consists of 11 T. urticae ABCGs, each having a maximum of 1 intron (see Additional file 3). Another T. urticae specific ABCG clade comprises 9 transporters of which 7 are located next to each other on scaffold 9. These seven ABCGs show high amino acid identity (between 50.8 and 91.7%) and have a conserved exon pattern (9 exons), indicating a common origin by successive tandem duplication events. Together with tetur06g05430 and tetur02g11270 they form a well-supported sister clade of D. melanogaster white and its D. pulex orthologues. Interestingly, no orthologues of D. melanogaster ABCGs brown and scarlet were found in T. urticae, while only one D. pulex orthologue of scarlet could be identified (Figure 4, [11]). About a century ago, the discovery of D. melanogaster white mutants with a remarkable eye-color phenotype marked the beginning of Drosophila genetics. As a consequence, D. melanogaster white is one of the most intensively studied fruit fly genes [99]. D. melanogaster white dimerises with either D. melanogaster scarlet or brown to form a transporter involved in the uptake of pigment precursors (guanine and tryptophan) in cells of developing compound and simple eyes [22]. T. urticae has, in contrast to D. pulex and D. melanogaster, no compound eyes and only four simple eyes (ocelli) [100]. Although no T. urticae orthologues of scarlet or brown were identified, dimerisation between the nine T. urticae co-orthologues of D. melanogaster white might result in a transporter capable of translocating pigment precursors into the cells of the spider mite ocelli (which have red pigment, as in D. melanogaster). However, these transporters might also have other functions besides transporting pigment precursors, as in other species roles have been documented in courtship behavior [101, 102], transport of biogenic amines [103] and uptake of uric acid [104] as was shown for D. melanogaster white and/or its B. mori orthologue.
Scaffold The Very Best Of Scaffold (2002).rar
We've seen the implementation of the original game's God AI system which still needs work but is becoming better with each release, scaffolding, creature coding and much more. But with so many systems comes a lot of work and promises were made. Unfortunately as a sole person on this project, I've been unable to complete the game fast enough to warrant release and in light of this, I wish to take OGaM in a new direction, something healthy for it's life and hopefully a boost to the community.
Hey everyone, I'm sorry this took so long to upload, but at long last I've decided to just post OGaM's full development cycle into one downloadable .rar file. If you wish to source these scripts for your own use, please do so, but be sure to credit where necessary. 2ff7e9595c
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